Pseudovirus Nanoparticles Displaying Plasmodium Circumsporozoite Proteins Elicited High Titers of Sporozoite-Binding Antibody.

ABSTRACT:

Background

malaria caused by Plasmodium parasites remains a public health threat. The circumsporozoite proteins (CSPs) of Plasmodium sporozoite play a key role in Plasmodium infection, serving as an excellent vaccine target.

Methods

using a self-assembled S₆₀ nanoparticle platform, we generated pseudovirus nanoparticles (PVNPs) displaying CSPs, named S-CSPs, for enhanced immunogenicity.

Results

purified Hisx6-tagged or tag-free S-CSPs self-assembled into PVNPs that consist of a norovirus S₆₀ inner shell and multiple surface-displayed CSPs. The majority of the PVNPs measured ~27 nm with some size variations, and their three-dimensional structure was modeled. The PVNP-displayed CSPs retained their glycan receptor-binding functi

SUBMITTER: Xia M 

PROVIDER: S-EPMC10674615 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature