Novel P335-like Phage Resistance Arises from Deletion within Putative Autolysin yccB in Lactococcus lactis.

ABSTRACT: Lactococcus lactis and Lactococcus cremoris are broadly utilized as starter cultures for fermented dairy products and are inherently impacted by bacteriophage (phage) attacks in the industrial environment. Consequently, the generation of bacteriophage-insensitive mutants (BIMs) is a standard approach for addressing phage susceptibility in dairy starter strains. In this study, we characterized spontaneous BIMs of L. lactis DGCC12699 that gained resistance against homologous P335-like phages. Phage resistance was found to result from mutations in the YjdB domain of yccB, a putative autolysin gene. We further observed that alteration of a fused tail-associated lysin-receptor binding protein (Tal-RBP) in the phage restored infectivity on the yccB BIMs. Additi

SUBMITTER: Seiler J 

PROVIDER: S-EPMC10675428 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature