Development and evaluation of specific polymerase chain reaction assays for detecting Theileria equi genotypes.

ABSTRACT:

Background

Theileria equi causes equine piroplasmosis, an economically significant disease that affects horses and other equids worldwide. Based on 18S ribosomal RNA (18S rRNA sequences), T. equi can be classified into five genotypes: A, B, C, D, and E. These genotypes have implications for disease management and control. However, no conventional polymerase chain reaction (PCR) assays are available to differentiate the genotypes of T. equi. To overcome this limitation, we developed and evaluated PCR assays specific for the detection of each T. equi genotype.

Methods

A pair of forward and reverse primers, specifically targeting the 18S rRNA sequence of each genotype, was designed. The genotype-specific PCR assays were evaluated for their specificity using plasmids containing

SUBMITTER: Ahedor B 

PROVIDER: S-EPMC10675911 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature