Paneth cell-derived iNOS is required to maintain homeostasis in the intestinal stem cell niche.

ABSTRACT:

Background

Mammalian intestinal epithelium constantly undergoes rapid self-renewal and regeneration sustained by intestinal stem cells (ISCs) within crypts. Inducible nitric oxide synthase (iNOS) is an important regulator in tissue homeostasis and inflammation. However, the functions of iNOS on ISCs have not been clarified. Here, we aimed to investigate the expression pattern of inducible nitric oxide synthase (iNOS) within crypts and explore its function in the homeostatic maintenance of the ISC niche.

Methods

Expression of iNOS was determined by tissue staining and qPCR. iNOS^-/- and Lgr5 transgenic mice were used to explore the influence of iNOS ablation on ISC proliferation and differentiation. Enteroids were cultured to study the effect of iNOS on ISCs in vitr

SUBMITTER: Huang L 

PROVIDER: S-EPMC10675917 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature