Evidence for widespread existence of functional novel and non-canonical human transcripts.

ABSTRACT:

Background

Fraction of functional sequence in the human genome remains a key unresolved question in Biology and the subject of vigorous debate. While a plethora of studies have connected a significant fraction of human DNA to various biochemical processes, the classical definition of function requires evidence of effects on cellular or organismal fitness that such studies do not provide. Although multiple high-throughput reverse genetics screens have been developed to address this issue, they are limited to annotated genomic elements and suffer from non-specific effects, arguing for a strong need to develop additional functional genomics approaches.

Results

In this work, we established a high-throughput lentivirus-based insertional mutagenesis strategy as a forward genetics

SUBMITTER: Xu D 

PROVIDER: S-EPMC10675921 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature