Identification of stably expressed microRNAs in plasma from high-grade serous ovarian carcinoma and benign tumor patients.

ABSTRACT:

Background

Ovarian cancer is a lethal gynecological cancer and no reliable minimally invasive early diagnosis tools exist. High grade serous ovarian carcinoma (HGSOC) is often diagnosed at advanced stages, resulting in poorer outcome than those diagnosed in early stage. Circulating microRNAs have been investigated for their biomarker potential. However, due to lack of standardization methods for microRNA detection, there is no consensus, which microRNAs should be used as stable endogenous controls. We aimed to identify microRNAs that are stably expressed in plasma of HGSOC and benign ovarian tumor patients.

Methods and results

We isolated RNA from plasma samples of 60 HGSOC and 48 benign patients. RT-qPCR was accomplished with a custom panel covering 40 microRNAs and 8 contr

SUBMITTER: Petersen PHD 

PROVIDER: S-EPMC10676310 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature