Assessment of hypoxia and oxidative-related changes in a lung-derived brain metastasis model by [⁶⁴Cu][Cu(ATSM)] PET and proteomic studies.

ABSTRACT:

Background

Brain metastases (BM) are the most frequent malignant brain tumors. The aim of this study was to characterize the tumor microenvironment (TME) of BM and particularly hypoxia and redox state, known to play a role in tumor growth and treatment resistance with multimodal PET and MRI imaging, immunohistochemical and proteomic approaches in a human lung cancer (H2030-BrM3)-derived BM model in rats.

Results

First, in vitro studies confirmed that H2030-BrM3 cells respond to hypoxia with increasing expression of HIF-1, HIF-2 and their target genes. Proteomic analyses revealed, among expression changes, proteins associated with metabolism, oxidative stress, metal response and hypoxia signaling in particular in cortical BM. [⁶⁴Cu][Cu(ATSM)] PET revealed a signifi

SUBMITTER: Fantin J 

PROVIDER: S-EPMC10676347 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature