An enhancer located in a Pde6c intron drives transient expression in the cone photoreceptors of developing mouse and human retinas.

ABSTRACT: How cone photoreceptors are formed during retinal development is only partially known. This is in part because we do not fully understand the gene regulatory network responsible for cone genesis. We reasoned that cis-regulatory elements (enhancers) active in nascent cones would be regulated by the same upstream network that controls cone formation. To dissect this network, we searched for enhancers active in developing cones. By electroporating enhancer-driven fluorescent reporter plasmids, we observed that a sequence within an intron of the cone-specific Pde6c gene acted as an enhancer in developing mouse cones. Similar fluorescent reporter plasmids were used to generate stable transgenic human induced pluripotent stem cells that were then grown into three-dimensional human retinal organo

SUBMITTER: Bachu VS 

PROVIDER: S-EPMC10676565 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature