Lung cDC1 and cDC2 dendritic cells priming naive CD8⁺ T cells in situ prior to migration to draining lymph nodes.

ABSTRACT: The current paradigm indicates that naive T cells are primed in secondary lymphoid organs. Here, we present evidence that intranasal administration of peptide antigens appended to nanofibers primes naive CD8⁺ T cells in the lung independently and prior to priming in the draining mediastinal lymph node (MLN). Notably, comparable accumulation and transcriptomic responses of CD8⁺ T cells in lung and MLN are observed in both Batf3KO and wild-type (WT) mice, indicating that, while cDC1 dendritic cells (DCs) are the major subset for cross-presentation, cDC2 DCs alone are capable of cross-priming CD8⁺ T cells both in the lung and draining MLN. Transcription analyses reveal distinct transcriptional responses in lung cDC1 and cDC2 to intranasal nanofiber immunizatio

SUBMITTER: Si Y 

PROVIDER: S-EPMC10676754 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature