Bioinformatics study of phytase from Aspergillus niger for use as feed additive in livestock feed.

ABSTRACT:

Background

Phytase supplementation in rations can reduce their phytic acid composition in order to enhance their nutritional value. Aspergillus niger is a fungus that can encode phytase. This study aims to determine the characteristics of its DNA sequences and amino acid composition that encode the phytase enzyme, as well as to determine the primer designs.

Method

This study used gene sequence data and protein-encoding phytase from Aspergillus niger that was collected manually from NCBI and PDB. The data was analyzed using SPDBV and then be aligned using the ClustalW Multiple Alignment features. The phylogenetic tree was built by Mega11 software. Primers were designed from selected candidate sequences that were analyzed. The designed primers were then simulated for PCR using

SUBMITTER: Maulana H 

PROVIDER: S-EPMC10678861 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature