One hundred single-copy nuclear sequence markers for olive variety identification: a case of fingerprinting database construction in China.

ABSTRACT: Olive is an ancient oil-producing tree, widely cultivated in Mediterranean countries, and now spread to other areas of the world, including China. Recently, several molecular databases were constructed in different countries and platforms for olive identification using simple sequence repeats (SSRs) or single-nucleotide polymorphisms (SNPs). However, comparing their results across laboratories was difficult. Herein, hundreds of polymorphic single-copy nuclear sequence markers were developed from the olive genome. Using the advantage of multiplex PCR amplification and high-throughput sequencing, a fingerprint database was constructed for the majority of olives cultivated in China. We used 100 high-quality sequence loci and estimated the genetic diversity and structure among all these variet

SUBMITTER: Huang L 

PROVIDER: S-EPMC10678893 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature