Spatiotemporal analysis of 3D human iPSC-derived neural networks using a 3D multi-electrode array.

ABSTRACT: While there is a growing appreciation of three-dimensional (3D) neural tissues (i.e., hydrogel-based, organoids, and spheroids), shown to improve cellular health and network activity to mirror brain-like activity in vivo, functional assessment using current electrophysiology techniques (e.g., planar multi-electrode arrays or patch clamp) has been technically challenging and limited to surface measurements at the bottom or top of the 3D tissue. As next-generation MEAs, specifically 3D MEAs, are being developed to increase the spatial precision across all three dimensions (X, Y, Z), development of improved computational analytical tools to discern region-specific changes within the Z dimension of the 3D tissue is needed. In the present study, we introduce a novel computational analyti

SUBMITTER: Lam D 

PROVIDER: S-EPMC10679684 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature