Multi-omics analyses reveal spatial heterogeneity in primary and metastatic oesophageal squamous cell carcinoma.

ABSTRACT:

Background

Biopsies obtained from primary oesophageal squamous cell carcinoma (ESCC) guide diagnosis and treatment. However, spatial intra-tumoral heterogeneity (ITH) influences biopsy-derived information and patient responsiveness to therapy. Here, we aimed to elucidate the spatial ITH of ESCC and matched lymph node metastasis (LN_met ).

Methods

Primary tumour superficial (PT_sup ), deep (PT_deep ) and LN_met subregions of patients with locally advanced resectable ESCC were evaluated using whole-exome sequencing (WES), whole-transcriptome sequencing and spatially resolved digital spatial profiling (DSP). To validate the findings, immunohistochemistry was conducted and a single-cell transcriptomic dataset was analysed.

Results

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SUBMITTER: Huang H 

PROVIDER: S-EPMC10679972 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature