The embryonic zebrafish brain is seeded by a lymphatic-dependent population of mrc1⁺ microglia precursors.

ABSTRACT: Microglia are the resident macrophages of the CNS that serve critical roles in brain construction. Although human brains contain microglia by 4 weeks gestation, an understanding of the earliest microglia that seed the brain during its development remains unresolved. Using time-lapse imaging in zebrafish, we discovered a mrc1a⁺ microglia precursor population that seeds the brain before traditionally described microglia. These early microglia precursors are dependent on lymphatic vasculature that surrounds the brain and are independent of pu1⁺ yolk sac-derived microglia. Single-cell RNA-sequencing datasets reveal Mrc1⁺ microglia in the embryonic brains of mice and humans. We then show in zebrafish that these early mrc1a⁺ microglia precursors prefer

SUBMITTER: Green LA 

PROVIDER: S-EPMC10680068 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature