Isolation and characterization of human periodontal ligament stem cells under the terms of use in clinical application: A pilot study.

ABSTRACT:

Background

The aim of the present study is to determine the possibility of isolation and characterization of the human periodontal ligament stem cells (hPDLSCs) using limited harvested periodontal ligament (PDL) tissue of only one patient's wisdom teeth (2-4 teeth) under the more compatible terms of use in clinical application without using the fetal bovine serum (FBS).

Materials and methods

In this pilot study, hPDLSCs were isolated from the impacted third molar, and tissue was scraped from the roots of the impacted third molar of 10 volunteers to enzymatically digest using collagenase. The cells were sub-cultured. The samples of the first seven patients and half of the eighth patient's sample were cultured in alpha modified of Eagle's medium (α-MEM) (-FBS) medium and the o

SUBMITTER: Behfarnia P 

PROVIDER: S-EPMC10680072 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature