Function and regulation of miR-186-5p, miR-125b-5p and miR-1260a in chordoma.

ABSTRACT:

Background

The function and regulation of miRNAs in progression of chordoma were unclear.

Methods

Five miRNAs were identified by the machine learning method from the miRNA expression array. CCk-8 assay, EDU assay, wound healing migration assay, and trans-well assay were used to reveal the effect of the miRNAs in chordoma cell lines. Moreover, bioinformation analysis and the mRNA expression array between the primary chordomas and recurrent chordomas were used to find the target protein genes of miRNAs. Furthermore, qRT-PCR and luciferase reporter assay were used to verify the result.

Results

miR-186-5p, miR-30c-5p, miR-151b, and miR-125b-5p could inhibit proliferation, migration, and invasion of chordoma while miR-1260a enhances proliferation, migration, and invasion o

SUBMITTER: Huo X 

PROVIDER: S-EPMC10680222 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature