Macrophage polarization toward M1 phenotype in T cell transfer colitis model.

ABSTRACT:

Background

T cell transfer colitis model is often used to study the CD4⁺ T cell functions in the intestine. However, the specific roles of macrophages in colitis remain unclear. In this study, we aimed to evaluate the phenotype and functions of macrophages in the colonic lamina propria (LP) in a colitis model.

Methods

Colitis was induced in scid mice via the adaptive transfer of CD4⁺CD45RB^hi T cells. Then, flow cytometry was used to determine the number of macrophages in the colonic LP and expression of cytokines in macrophages at the onset of colitis. Moreover, M1/M2 macrophage markers were detected in the colonic LP during colitis development using high-dimensional single-cell data and gating-based analyses. Expression levels of M1 markers

SUBMITTER: Ebihara S 

PROVIDER: S-EPMC10680295 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature