Mild heat treatment in vitro potentiates human adipose stem cells: delayed aging and improved quality for long term culture.

ABSTRACT:

Background

Mesenchymal stem cells (MSCs) have gained significant attention for diverse biomedical applications, including cell-based therapy. Hence, in vitro expansion of MSCs is critical; however, in vitro MSC culture, especially long-term culture, inevitably leads to significant loss of stemness, growth, and differentiation potential.

Method

Effects of mild heat treatment (HT) conditions (temperature, duration, and repetition) on the characteristics of adipose tissue-derived MSCs in vitro were systematically investigated. Characteristics of the MSCs subjected to the predetermined HT conditions (41 or 44ºC, 1 h, and 2X HT) were first analyzed in a single passage using various assays. In addition, the feasibility of HT for long-term MSC culture was studied. The RNA sequencin

SUBMITTER: Ryu C 

PROVIDER: S-EPMC10680349 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature