Rh flow cytometry: An updated methodology for D antigen density applied to weak D types 164 and 165.

ABSTRACT:

Background

An original methodology for determining the D antigen density on red cells was published in 2000 and has been applied in many publications since. This flow cytometry-based assay remained largely unrevised utilizing monoclonal anti-Ds that are not readily available anymore. We updated the methodology to quantify erythrocyte D antigen sites using microspheres and monoclonal anti-Ds that are commercially available today.

Methods

The absolute D antigen density of a frozen standard CcDEe cell, drawn in 2003, a fresh blood donation from the same individual, drawn in 2022, and an internal control CcDEe cell, was quantified by flow cytometry using fluorescence-labeled microspheres. The internal control CcDEe cell was used in conjunction with 9 commercial anti-Ds to determ

SUBMITTER: Hutchison CJ 

PROVIDER: S-EPMC10680490 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature