Asthma-protective agents in dust from traditional farm environments.

ABSTRACT:

Background

Growing up on traditional European or US Amish dairy farms in close contact with cows and hay protects children against asthma, and airway administration of extracts from dust collected from cowsheds of those farms prevents allergic asthma in mice.

Objectives

This study sought to begin identifying farm-derived asthma-protective agents.

Methods

Our work unfolded along 2 unbiased and independent but complementary discovery paths. Dust extracts (DEs) from protective and nonprotective farms (European and Amish cowsheds vs European sheep sheds) were analyzed by comparative nuclear magnetic resonance profiling and differential proteomics. Bioactivity-guided size fractionation focused on protective Amish cowshed DEs. Multiple in vitro and in vivo functional assays

SUBMITTER: Marques Dos Santos M 

PROVIDER: S-EPMC10680491 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature