The role of BST4 in the pyrenoid of <i>Chlamydomonas reinhardtii</i>.

ABSTRACT: In many eukaryotic algae, CO₂ fixation by Rubisco is enhanced by a CO₂-concentrating mechanism, which utilizes a Rubisco-rich organelle called the pyrenoid. The pyrenoid is traversed by a network of thylakoid-membranes called pyrenoid tubules, proposed to deliver CO₂. In the model alga Chlamydomonas reinhardtii (Chlamydomonas), the pyrenoid tubules have been proposed to be tethered to the Rubisco matrix by a bestrophin-like transmembrane protein, BST4. Here, we show that BST4 forms a complex that localizes to the pyrenoid tubules. A Chlamydomonas mutant impaired in the accumulation of BST4 (bst4) formed normal pyrenoid tubules and heterologous expression of BST4 in Arabidopsis thaliana did not lead to the incorporation of

SUBMITTER: Adler L 

PROVIDER: S-EPMC10680556 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature