Three-dimensional reconstruction of interstitial extracellular vesicles in human liver as determined by electron tomography.

ABSTRACT: Extracellular vesicles (EVs) are lipid bilayer nanoparticles involved in cell-cell communication that are released into the extracellular space by all cell types. The cargo of EVs includes proteins, lipids, nucleic acids, and metabolites reflecting their cell of origin. EVs have recently been isolated directly from solid tissues, and this may provide insights into how EVs mediate communication between cells in vivo. Even though EVs have been isolated from tissues, their point of origin when they are in the interstitial space has been uncertain. In this study, we performed three-dimensional (3D) reconstruction using transmission electron tomography of metastatic and normal liver tissues with a focus on the presence of EVs in the interstitium. After chemical fixation of the samples and subse

SUBMITTER: Olofsson Bagge R 

PROVIDER: S-EPMC10680575 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature