Thyroid hormone increases fatty acid use in fetal ovine cardiac myocytes.

ABSTRACT: Cardiac metabolic substrate preference shifts at parturition from carbohydrates to fatty acids. We hypothesized that thyroid hormone (T₃ ) and palmitic acid (PA) stimulate fetal cardiomyocyte oxidative metabolism capacity. T₃ was infused into fetal sheep to a target of 1.5 nM. Dispersed cardiomyocytes were assessed for lipid uptake and droplet formation with BODIPY-labeled fatty acids. Myocardial expression levels were assessed PCR. Cardiomyocytes from naïve fetuses were exposed to T₃ and PA, and oxygen consumption was measured with the Seahorse Bioanalyzer. Cardiomyocytes (130-day gestational age) exposed to elevated T₃ in utero accumulated 42% more long-chain fatty acid droplets than did cells from vehicle-infused fetuses. In utero T₃

SUBMITTER: Chattergoon N 

PROVIDER: S-EPMC10680578 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature