The human Toll-like receptor 2 (TLR2) response during pathogenic Leptospira infection.

ABSTRACT:

Background

Human innate immune responses are triggered through the interaction of human pattern recognition receptors and pathogen-associated molecular patterns. The role of toll-like receptor2 (TLR2) in mice innate immune response to leptospirosis is well established, while human studies are limited. The present study aimed to determine the TLR2 response among confirmed cases of leptospirosis.

Methodology/principle findings

The study has two components. Clinically suspected patients of leptospirosis were confirmed using a previously validated qPCR assay. Total RNA was extracted from patients' RNA-stabilized whole blood samples. Human TLR2 gene expression (RT-qPCR) analysis was carried out using an exon-exon spanning primer pair, using CFX Maestro^™ software. The f

SUBMITTER: Kappagoda CN 

PROVIDER: S-EPMC10680769 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature