A Large Field-of-view, Single-cell-resolution Two- and Three-Photon Microscope for Deep Imaging.

ABSTRACT: In vivo imaging of large-scale neuron activity plays a pivotal role in unraveling the function of the brain's network. Multiphoton microscopy, a powerful tool for deep-tissue imaging, has received sustained interest in advancing its speed, field of view and imaging depth. However, to avoid thermal damage in scattering biological tissue, field of view decreases exponentially as imaging depth increases. We present a suite of innovations to overcome constraints on the field of view in three-photon microscopy and to perform deep imaging that is inaccessible to two-photon microscopy. These innovations enable us to image neuronal activities in a ~3.5-mm diameter field-of-view at 4 Hz with single-cell resolution and in the deepest cortical layer of mouse brains. We further demonstrate simultaneou

SUBMITTER: Mok AT 

PROVIDER: S-EPMC10680773 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature