Cell morphology QTL reveal gene by environment interactions in a genetically diverse cell population.

ABSTRACT: Genetically heterogenous cell lines from laboratory mice are promising tools for population-based screening as they offer power for genetic mapping, and potentially, predictive value for in vivo experimentation in genetically matched individuals. To explore this further, we derived a panel of fibroblast lines from a genetic reference population of laboratory mice (the Diversity Outbred, DO). We then used high-content imaging to capture hundreds of cell morphology traits in cells exposed to the oxidative stress-inducing arsenic metabolite monomethylarsonous acid (MMA^III). We employed dose-response modeling to capture latent parameters of response and we then used these parameters to identify several hundred cell morphology quantitative trait loci (cmQTL). Response cmQTL en

SUBMITTER: O'Connor C 

PROVIDER: S-EPMC10680806 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature