A simeprevir-inducible molecular switch for the control of cell and gene therapies.

ABSTRACT: Chemical inducer of dimerization (CID) modules can be used effectively as molecular switches to control biological processes, and thus there is significant interest within the synthetic biology community in identifying novel CID systems. To date, CID modules have been used primarily in engineering cells for in vitro applications. To broaden their utility to the clinical setting, including the potential to control cell and gene therapies, the identification of novel CID modules should consider factors such as the safety and pharmacokinetic profile of the small molecule inducer, and the orthogonality and immunogenicity of the protein components. Here we describe a CID module based on the orally available, approved, small molecule simeprevir and its target, the NS3/4A protease from hepatitis

SUBMITTER: Chin SE 

PROVIDER: S-EPMC10682029 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature