The oxygen-tolerant reductive glycine pathway assimilates methanol, formate and CO₂ in the yeast Komagataella phaffii.

ABSTRACT: The current climatic change is predominantly driven by excessive anthropogenic CO₂ emissions. As industrial bioprocesses primarily depend on food-competing organic feedstocks or fossil raw materials, CO₂ co-assimilation or the use of CO₂-derived methanol or formate as carbon sources are considered pathbreaking contributions to solving this global problem. The number of industrially-relevant microorganisms that can use these two carbon sources is limited, and even fewer can concurrently co-assimilate CO₂. Here, we search for alternative native methanol and formate assimilation pathways that co-assimilate CO₂ in the industrially-relevant methylotrophic yeast Komagataella phaffii (Pichia pastoris). Using ¹³C-tracer-based metabo

SUBMITTER: Mitic BM 

PROVIDER: S-EPMC10682033 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature