Role of histamine-mediated macrophage differentiation in clearance of metastatic bacterial infection.

ABSTRACT: Macrophages are highly heterogeneous immune cells with a role in maintaining tissue homeostasis, especially in activating the defense response to bacterial infection. Using flow cytometric and single-cell RNA-sequencing analyses of peritoneal cells, we here show that small peritoneal macrophage and immature macrophage populations are enriched in histamine-deficient (Hdc ^-/-) mice, characterized by a CD11b^miF4/80^loCCR2⁺MHCII^hi and CD11b^loF4/80^miTHBS1⁺IL-1α⁺ phenotype, respectively. Molecular characterization revealed that immature macrophages represent an abnormally differentiated form of large peritoneal macrophages with strong inflammatory properties. Furthermore, deficiency in histam

SUBMITTER: Kim KH 

PROVIDER: S-EPMC10682073 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature