An in vitro model system based on calcium- and phosphate ion-induced hMSC spheroid mineralization.

ABSTRACT: A challenge in regenerative medicine is creating the three-dimensional organic and inorganic in vitro microenvironment of bone, which would allow the study of musculoskeletal disorders and the generation of building blocks for bone regeneration. This study presents a microwell-based platform for creating spheroids of human mesenchymal stromal cells, which are then mineralized using ionic calcium and phosphate supplementation. The resulting mineralized spheroids promote an osteogenic gene expression profile through the influence of the spheroids' biophysical environment and inorganic signaling and require less calcium or phosphate to achieve mineralization compared to a monolayer culture. We found that mineralized spheroids represent an in vitro model for studying small molecu

SUBMITTER: Vermeulen S 

PROVIDER: S-EPMC10682137 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature