Dataset Information

Vascular adhesion protein-1-targeted PET imaging in autoimmune myocarditis.

ABSTRACT:

Background

Vascular adhesion protein-1 (VAP-1) is an adhesion molecule and primary amine oxidase, and Gallium-68-labeled 1,4,7,10-tetraazacyclododecane-N,N',N″,N‴-tetra-acetic acid conjugated sialic acid-binding immunoglobulin-like lectin 9 motif containing peptide ([⁶⁸Ga]Ga-DOTA-Siglec-9) is a positron emission tomography (PET) tracer targeting VAP-1. We evaluated the feasibility of PET imaging with [⁶⁸Ga]Ga-DOTA-Siglec-9 for the detection of myocardial lesions in rats with autoimmune myocarditis.

Methods

Rats (n = 9) were immunized twice with porcine cardiac myosin in complete Freund's adjuvant. Control rats (n = 6) were injected with Freund's adjuvant alone. On day 21, in vivo PET/computed tomography (CT) imaging with [⁶⁸Ga]Ga-DOTA-Siglec-9 was performed, followed by ex vivo autoradiography, histology, and immunohistochemistry of tissue sections. In addition, myocardial samples from three patients with cardiac sarcoidosis were studied.

Results

[⁶⁸Ga]Ga-DOTA-Siglec-9 PET/CT images of immunized rats showed higher uptake in myocardial lesions than in myocardium outside lesions (SUV_mean, 0.5 ± 0.1 vs 0.3 ± 0.1; P = .003) or control rats (SUV_mean, 0.2 ± 0.03; P < .0001), which was confirmed by ex vivo autoradiography of tissue sections. Immunohistochemistry showed VAP-1-positive staining in lesions of rats with myocarditis and in patients with cardiac sarcoidosis.

Conclusion

VAP-1-targeted [⁶⁸Ga]Ga-DOTA-Siglec-9 PET is a potential novel technique for the detection of myocardial lesions.

SUBMITTER: Jahandideh A

PROVIDER: S-EPMC10682147 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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Publications

Vascular adhesion protein-1-targeted PET imaging in autoimmune myocarditis.

Jahandideh Arghavan A Virta Jenni J Li Xiang-Guo XG Liljenbäck Heidi H Moisio Olli O Ponkamo Jesse J Rajala Noora N Alix Marion M Lehtonen Jukka J Mäyränpää Mikko I MI Salminen Tiina A TA Knuuti Juhani J Jalkanen Sirpa S Saraste Antti A Roivainen Anne A

Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology 20230927 6

<h4>Background</h4>Vascular adhesion protein-1 (VAP-1) is an adhesion molecule and primary amine oxidase, and Gallium-68-labeled 1,4,7,10-tetraazacyclododecane-N,N',N″,N‴-tetra-acetic acid conjugated sialic acid-binding immunoglobulin-like lectin 9 motif containing peptide ([<sup>68</sup>Ga]Ga-DOTA-Siglec-9) is a positron emission tomography (PET) tracer targeting VAP-1. We evaluated the feasibility of PET imaging with [<sup>68</sup>Ga]Ga-DOTA-Siglec-9 for the detection of myocardial lesions in ...[more]

PMID: 37758963

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Dataset Information

Vascular adhesion protein-1-targeted PET imaging in autoimmune myocarditis.

Background

Methods

Results

Conclusion

Publications

Vascular adhesion protein-1-targeted PET imaging in autoimmune myocarditis.

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