Endocytosis inhibitors block SARS-CoV-2 pseudoparticle infection of mink lung epithelium.

ABSTRACT:

Introduction

Both spill over and spill back of SARS-CoV-2 virus have been reported on mink farms in Europe and the United States. Zoonosis is a public health concern as dangerous mutated forms of the virus could be introduced into the human population through spillback.

Methods

The purpose of our study was to determine the SARS-CoV-2 entry mechanism using the mink lung epithelial cell line (Mv1Lu) and to block entry with drug inhibitors.

Results

Mv1Lu cells were susceptible to SARS-CoV-2 viral pseudoparticle infection, validating them as a suitable disease model for COVID-19. Inhibitors of TMPRSS2 and of endocytosis, two pathways of viral entry, were tested to identify those that blocked infection. TMPRSS2 inhibitors had minimal impact, which can be explained by the a

SUBMITTER: Song A 

PROVIDER: S-EPMC10682793 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature