Activation dynamics of antigen presenting cells in vivo against Mycobacterium bovis BCG in different immunized route.

ABSTRACT:

Background

Control of Tuberculosis (TB) infection is mainly the result of productive teamwork between T-cell populations and antigen presenting cells (APCs). However, APCs activation at the site of initiating cellular immune response during BCG early infection is not completely understood.

Methods

In this study, we injected C57BL/6 mice in intravenous (i.v) or subcutaneous (s.c) route, then splenic or inguinal lymph node (LN) DCs and MΦs were sorted, and mycobacteria uptake, cytokine production, antigen presentation activity, and cell phenotype were investigated and compared, respectively.

Results

Ag85A-specific T-cell immune response began at 6 days post BCG infection, when BCG was delivered in s.c route, Th17 immune response could be induced in inguinal LN. BCG coul

SUBMITTER: Xu Z 

PROVIDER: S-EPMC10683112 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature