Transplantation of active nucleus pulposus cells with a keep-charging hydrogel microsphere system to rescue intervertebral disc degeneration.

ABSTRACT:

Background

Cell transplantation has been demonstrated as a promising approach in tissue regeneration. However, the reactive oxygen species (ROS) accumulation and inflammation condition establish a harsh microenvironment in degenerated tissue, which makes the transplanted cells difficult to survive.

Methods

In this study, we constructed a keep-charging hydrogel microsphere system to enable cells actively proliferate and function in the degenerated intervertebral disc. Specifically, we combined Mg²⁺ to histidine-functionalized hyaluronic acid (HA-His-Mg²⁺) through coordination reaction, which was further intercrossed with GelMA to construct a double-network hydrogel microsphere (GelMA/HA-His-Mg²⁺, GHHM) with microfluidic methods. In vitro, the

SUBMITTER: Tang Y 

PROVIDER: S-EPMC10683266 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature