Oral microbiota analyses of paediatric Saudi population reveals signatures of dental caries.

ABSTRACT:

Background

Oral microbiome sequencing has revealed key links between microbiome dysfunction and dental caries. However, these efforts have largely focused on Western populations, with few studies on the Middle Eastern communities. The current study aimed to identify the composition and abundance of the oral microbiota in saliva samples of children with different caries levels using machine learning approaches.

Methods

Oral microbiota composition and abundance were identified in 250 Saudi participants with high dental caries and 150 with low dental caries using 16 S rRNA sequencing on a NextSeq 2000 SP flow cell (Illumina, CA) using 250 bp paired-end reads, and attempted to build a classifier using random forest models to assist in the early detection of caries.

Results

SUBMITTER: Alyousef YM 

PROVIDER: S-EPMC10683298 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature