Dataset Information

Identification and validation of ubiquitination-related signature and subgroups in immune microenvironment of tuberculosis.

ABSTRACT:

Background

Mycobacterium tuberculosis (Mtb) is the bacterial pathogen responsible for causing tuberculosis (TB), a severe public health concern that results in numerous deaths worldwide. Ubiquitination (Ub) is an essential physiological process that aids in maintaining homeostasis and contributes to the development of TB. Therefore, the main objective of our study was to investigate the potential role of Ub-related genes in TB.

Methods

Our research entailed utilizing single sample gene set enrichment analysis (ssGSEA) in combination with several machine learning techniques to discern the Ub-related signature of TB and identify potential diagnostic markers that distinguish TB from healthy controls (HC).

Results

In summary, we used the ssGSEA algorithm to determine the score of Ub families (E1, E2, E3, DUB, UBD, and ULD). Notably, the score of E1, E3, and UBD were lower in TB patients than in HC individuals, and we identified 96 Ub-related differentially expressed genes (UbDEGs). Employing machine learning algorithms, we identified 11 Ub-related hub genes and defined two distinct Ub-related subclusters. Notably, through GSVA and functional analysis, it was determined that these subclusters were implicated in numerous immune-related processes. We further investigated these Ub-related hub genes in four TB-related diseases and found that TRIM68 exhibited higher correlations with various immune cells in different conditions, indicating that it may play a crucial role in the immune process of these diseases.

Conclusion

The observed enrichment of Ub-related gene expression in TB patients emphasizes the potential involvement of ubiquitination in the progression of TB. These significant findings establish a basis for future investigations to elucidate the molecular mechanisms associated with TB, select suitable diagnostic biomarkers, and design innovative therapeutic interventions for combating this fatal infectious disease.

SUBMITTER: Zhou P

PROVIDER: S-EPMC10683621 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature

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Publications

Identification and validation of ubiquitination-related signature and subgroups in immune microenvironment of tuberculosis.

Zhou Peipei P Shen Jie J Ge Xiao X Cheng Haien H Sun Yanli Y Li Meng M Li Heng H Yi Zhengjun Z Li Zhenpeng Z

Aging 20231109 21

<h4>Background</h4><i>Mycobacterium tuberculosis</i> (Mtb) is the bacterial pathogen responsible for causing tuberculosis (TB), a severe public health concern that results in numerous deaths worldwide. Ubiquitination (Ub) is an essential physiological process that aids in maintaining homeostasis and contributes to the development of TB. Therefore, the main objective of our study was to investigate the potential role of Ub-related genes in TB.<h4>Methods</h4>Our research entailed utilizing single ...[more]

PMID: 37950733

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Dataset Information

Identification and validation of ubiquitination-related signature and subgroups in immune microenvironment of tuberculosis.

Background

Methods

Results

Conclusion

Publications

Identification and validation of ubiquitination-related signature and subgroups in immune microenvironment of tuberculosis.

Similar Datasets

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