Cuproptosis-related gene DLAT serves as a prognostic biomarker for immunotherapy in clear cell renal cell carcinoma: multi-database and experimental verification.

ABSTRACT:

Objective

Renal clear cell carcinoma (ccRCC) is the most common type of renal cancer. Here we aim to explore the prognosis and immunotherapeutic value of copper death-related gene Dihydrolipoamide S-acetyltransferase (DLAT) in ccRCC.

Methods

The mRNA and protein expressions and methylation level of DLAT, as well as the relation of DLAT to survival prognosis, clinical characteristics, biological function, and immune microenvironment and responses in patients with ccRCC were evaluated using multiple databases. In addition, 75 paired ccRCC tissue samples and 3 kinds of cell lines were tested for experimental validation.

Results

Bioinformatics analysis of multiple databases, qRT-PCR, and western blot verified that DLAT expression in ccRC

SUBMITTER: Huang S 

PROVIDER: S-EPMC10683628 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature