Field-based detection of bacteria using nanopore sequencing: Method evaluation for biothreat detection in complex samples.

ABSTRACT: From pathogen detection to genome or plasmid closure, the utility of the Oxford Nanopore Technologies (ONT) MinION for microbiological analysis has been well documented. The MinION's small footprint, portability, and real-time analytic capability situates it well to address challenges in the field of unbiased pathogen detection, as a component of a security investigation. To this end, a multicenter evaluation of the effect of alternative analytical approaches on the outcome of MinION-based sequencing, using a set of well-characterized samples, was explored in a field-based scenario. Three expert scientific response groups evaluated known bacterial DNA extracts as part of an international first responder (Chemical, Biological, Radiological) training exercise. Samples were prepared independe

SUBMITTER: Tyler AD 

PROVIDER: S-EPMC10684088 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature