On the interdependence of ketone body oxidation, glycogen content, glycolysis and energy metabolism in the heart.

ABSTRACT: In heart, glucose and glycolysis are important for anaplerosis and potentially therefore for d-β-hydroxybutyrate (βHB) oxidation. As a glucose store, glycogen may also furnish anaplerosis. We determined the effects of glycogen content on βHB oxidation and glycolytic rates, and their downstream effects on energetics, in the isolated rat heart. High glycogen (HG) and low glycogen (LG) containing hearts were perfused with 11 mM [5-³ H]glucose and/or 4 mM [¹⁴ C]βHB to measure glycolytic rates or βHB oxidation, respectively, then freeze-clamped for glycogen and metabolomic analyses. Free cytosolic [NAD⁺ ]/[NADH] and mitochondrial [Q⁺ ]/[QH₂ ] ratios were estimated using the lactate dehydrogenase and succinate dehydrogenase reaction, respect

SUBMITTER: Kadir AA 

PROVIDER: S-EPMC10684314 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature