Automated silylation of flavonoids using 3D printed microfluidics prior to chromatographic analysis: system development.

ABSTRACT: Flavonoids are a class of secondary plant metabolites with low molecular weights. Most flavonoids are highly polar and unsuitable for gas chromatographic analyses. Derivatization is commonly used to make them amenable to gas chromatography by altering their physicochemical properties. Although highly effective, derivatization techniques introduce extra preparation steps and often use hazardous chemicals. The aim of this study was to automate derivatization (specifically, silylation) by developing 3D printed microfluidic devices in which derivatization of flavonoids can occur. A microfluidic device was designed and 3D printed using clear polypropylene. Quercetin and other flavonoids (TED 13 and ZTF 1016) isolated from plant extracts were silylated with N-tert-butyldimethylsilyl-N-methyltrif

SUBMITTER: Ncongwane TB 

PROVIDER: S-EPMC10684624 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature