A comparative analysis of genes differentially expressed between rete testis cells and Sertoli cells of the mouse testis.

ABSTRACT: The rete testis (RT) is a region of the mammalian testis that plays an important role in testicular physiology. The RT epithelium consists of cells sharing some well-known gene markers with supporting Sertoli cells (SCs). However, little is known about the differences in gene expression between these two cell populations. Here, we used fluorescence-activated cell sorting (FACS) to obtain pure cultures of neonatal RT cells and SCs and identified differentially expressed genes (DEGs) between these cell types using RNA sequencing (RNA-seq). We then compared our data with the RNA-seq data of other studies that examined RT cells and SCs of mice of different ages and generated a list of DEGs permanently upregulated in RT cells throughout testis development and in culture, which included 86 genes

SUBMITTER: Malolina EA 

PROVIDER: S-EPMC10684643 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature