Liquid Biopsy in Organ Damage: small extracellular vesicle chip-based assessment of polytrauma.

ABSTRACT:

Background

Despite major advances in medicine, blood-borne biomarkers are urgently needed to support decision-making, including polytrauma. Here, we assessed serum-derived extracellular vesicles (EVs) as potential markers of decision-making in polytrauma.

Objective

Our Liquid Biopsy in Organ Damage (LiBOD) study aimed to differentiate polytrauma with organ injury from polytrauma without organ injury. We analysed of blood-borne small EVs at the individual level using a combination of immunocapture and high-resolution imaging.

Methods

To this end, we isolated, purified, and characterized small EVs according to the latest Minimal Information for Studies of Extracellular Vesicles (MISEV) guidelines from human blood collected within 24 h post-trauma and validated our resul

SUBMITTER: Wang B 

PROVIDER: S-EPMC10684673 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature