Interrogating ligand-receptor interactions using highly sensitive cellular biosensors.

ABSTRACT: Interactions of membrane-resident proteins are important targets for therapeutic interventions but most methods to study them are either costly, laborious or fail to reflect the physiologic interaction of membrane resident proteins in trans. Here we describe highly sensitive cellular biosensors as a tool to study receptor-ligand pairs. They consist of fluorescent reporter cells that express chimeric receptors harboring ectodomains of cell surface molecules and intracellular signaling domains. We show that a broad range of molecules can be integrated into this platform and we demonstrate its applicability to highly relevant research areas, including the characterization of immune checkpoints and the probing of cells for the presence of receptors or ligands. The platform is suitable to evalu

SUBMITTER: Funk MA 

PROVIDER: S-EPMC10684770 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature