Characterization of the TCRβ repertoire of peripheral MR1-restricted MAIT cells in psoriasis vulgaris patients.

ABSTRACT: Psoriasis vulgaris (PV) is an inflammatory skin disease largely driven by aberrant αβT cells. Mucosal-associated invariant T (MAIT) cells, which constitute the largest circulating innate-like αβT cell community in human adults, are characterized by a semi-invariant TCRVα7.2 receptor and MR1-restricted affinity toward microbial metabolites. Limited MAIT TCRα diversity is complemented by a more variable TCRβ repertoire, but its footprint in the MAIT repertoire of PV patients has never been tested. Here, we used bulk TCRSeq, MiXCR, VDJTools, and Immunarch pipelines to decipher and compare TCRβ clonotypes from flow-sorted, peripheral TCRVα7.2⁺MR1-5-OP-RU-tet⁺MAIT cells from 10 PV patients and 10 healthy, matched controls. The resulting TCRβ collections were highly private

SUBMITTER: Jirous Drulak M 

PROVIDER: S-EPMC10684872 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature