ITRAQ-based quantitative proteomic analysis of the antibacterial mechanism of silver nanoparticles against multidrug-resistant Streptococcus suis.

ABSTRACT:

Background

The increase in antibiotic resistance of bacteria has become a major concern in clinical treatment. Silver nanoparticles (AgNPs) have significant antibacterial effects against Streptococcus suis. Therefore, this study aimed to investigate the antibacterial activity and mechanism of action of AgNPs against multidrug-resistant S. suis.

Methods

The effect of AgNPs on the morphology of multidrug-resistant S. suis was observed using scanning electron microscopy (SEM). Differentially expressed proteins were analyzed by iTRAQ quantitative proteomics, and the production of reactive oxygen species (ROS) was assayed by H₂DCF-DA staining.

Results

SEM showed that AgNPs disrupted the normal morphology of multidrug-resistant S. suis

SUBMITTER: Liu B 

PROVIDER: S-EPMC10684948 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature