Combined Computational-Biochemical Approach Offers an Accelerated Path to Membrane Protein Solubilization.

ABSTRACT: Membrane proteins are difficult to isolate and purify due to their dependence on the surrounding lipid membrane for structural stability. Detergents are often used to solubilize these proteins, with this approach requiring a careful balance between protein solubilization and denaturation. Determining which detergent is most appropriate for a given protein has largely been done empirically through screening, which requires large amounts of membrane protein and associated resources. Here, we describe an alternative to conventional detergent screening using a computational modeling approach to identify the most likely candidate detergents for solubilizing a protein of interest. We demonstrate our approach using ghrelin O-acyltransferase (GOAT), a member of the membrane-bound O-a

SUBMITTER: Pierce MR 

PROVIDER: S-EPMC10685452 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature