High-throughput droplet microfluidics screening and genome sequencing analysis for improved amylase-producing Aspergillus oryzae.

ABSTRACT:

Background

The exceptional protein secretion capacity, intricate post-translational modification processes, and inherent safety features of A. oryzae make it a promising expression system. However, heterologous protein expression levels of existing A. oryzae species cannot meet the requirement for industrial-scale production. Therefore, establishing an efficient screening technology is significant for the development of the A. oryzae expression system.

Results

In this work, a high-throughput screening method suitable for A. oryzae has been established by combining the microfluidic system and flow cytometry. Its screening efficiency can reach 350 droplets per minute. The diameter of the microdroplet was enlarged to 290 µm to adapt to the polar growth of A. oryzae hyphae. Thro

SUBMITTER: Li Q 

PROVIDER: S-EPMC10685594 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature