Iron Oxide Nanoparticles Conjugated to Thiosemicarbazone Reduce the Survival of Cancer Cells by Increasing the Gene Expression of MicroRNA let-7c in Lung Cancer A549 Cells.

ABSTRACT:

Background

Cancer cells have a higher demand for iron to grow and proliferate. A new complex of iron nanoparticles and thiosemicarbazones was synthesized. Confirmation tests included UV-visible, scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), Fourier transform infrared (FTIR), X-ray diffraction (XRD) and zeta potential.

Methods

MTT assay, flow cytometry and qRT-PCR were used to investigate anti-proliferative effect, amount of apoptosis and the effect of Fe₃ O₄ @Glu/BTSC on changes in gene expression of microRNA let-7c (let-7c), respectively. The specifications of Fe₃ O₄ @ Glu/BTSC were confirmed at 5 nm.

Results

Fe3O4@Glu/BTSC was more effective than BTSC and Fe₃ O_4</

SUBMITTER: Habibi A 

PROVIDER: S-EPMC10685841 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature