On-capillary alkylation micro-reactor: a facile strategy for proteo-metabolome profiling in the same single cells.

ABSTRACT: Single-cell multi-omics analysis can provide comprehensive insights to study cell-to-cell heterogeneity in normal and disease physiology. However, due to the lack of amplification technique, the measurement of proteome and metabolome in the same cell is challenging. Herein, a novel on-capillary alkylation micro-reactor (OCAM) was developed to achieve proteo-metabolome profiling in the same single cells, by which proteins were first covalently bound to an iodoacetic acid functionalized open-tubular capillary micro-reactor via sulfhydryl alkylation reaction, and metabolites were rapidly eluted, followed by on-column digestion of captured proteins. Compared with existing methods for low-input proteome sample preparation, OCAM exhibited improved efficiency, anti-interference ability and

SUBMITTER: He Y 

PROVIDER: S-EPMC10686037 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature