Dataset Information

intI1 gene abundance from septic tanks in Thailand using validated intI1 primers.

ABSTRACT:

Importance

Antimicrobial resistance is a global crisis, and wastewater treatment, including septic tanks, remains an important source of antimicrobial resistance (AMR) genes. The role of septic tanks in disseminating class 1 integron, and by extension AMR genes, in Thailand, where antibiotic use is unregulated remains understudied. We aimed to monitor gene abundance as a proxy to infer potential AMR from septic tanks in Thailand. We evaluated published intI1 primers due to the lack of consensus on optimal Q-PCR primers and the absence of standardization. Our findings confirmed septic tanks are a source of class 1 integron to the environment. We highlighted the significance of intI1 primer choice, in the context of interpretation of risk associated with AMR spread from septic tanks. We recommend the validated set (F3-R3) for optimal intI1 quantification toward the goal of achieving standardization across studies.

SUBMITTER: Okonkwo V

PROVIDER: S-EPMC10686061 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature

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Publications

<i>intI</i>1 gene abundance from septic tanks in Thailand using validated <i>intI</i>1 primers.

Okonkwo Valentine V Cholet Fabien F Ijaz Umer Z UZ Koottatep Thammarat T Pussayanavin Tatchai T Polpraset Chongrak C Sloan William T WT Connelly Stephanie S Smith Cindy J CJ

Applied and environmental microbiology 20231024 11

<h4>Importance</h4>Antimicrobial resistance is a global crisis, and wastewater treatment, including septic tanks, remains an important source of antimicrobial resistance (AMR) genes. The role of septic tanks in disseminating class 1 integron, and by extension AMR genes, in Thailand, where antibiotic use is unregulated remains understudied. We aimed to monitor gene abundance as a proxy to infer potential AMR from septic tanks in Thailand. We evaluated published <i>intI</i>1 primers due to the lac ...[more]

PMID: 37874304

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